Jiřina Bartůňková graduated from the Faculty of General Medicine of Charles University where she later worked in the clinical immunology unit.
She received her postgraduate certification in internal medicine and her specialist certification in clinical immunology and allergology. She was appointed Professor of Immunology in 2003. She took several fellowships at leading immunology institutions outside the Czech Republic.
In addition to practicing medicine, she is engaged in research and teaching in the field of clinical immunology. She is Head of the Department of Immunology of the 2nd Faculty of Medicine of Charles University, Prague. She initiated and lead the dendritic-cell based anti-cancer vaccine development from preclinical research until phase III clinical trials.